IRES-coordinated Translation Model

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Model for HCV IRES-coordinated assembly of human translation initiation complexes. 40S subunit binding to IRES RNA with an intact IIId loop is required for IRES-40S association; eIF3 interaction with the IIIb region of the IRES is required for stable association of eIF3 and eIF2 in the 48S complex, whereas eIF3 contacts to the junction of stems IIIa, -b and -c, as well as the 40S conformational change induced by DomII, are necessary for downstream events required for 60S subunit joining.

Reference:

Ji, H., Fraser, C.S., Yu, Y., Leary, J. and Doudna, J.A. (2004) Coordinated assembly of human translation initiation complexes by the hepatitis C virus internal ribosome entry site RNA. PNAS 101, 16990-16995. (508KB PDF)