SRP - FtsY Model
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| Click on the image to see the full-size view (1.6MB .jpg) | Click on the image to see the full-size view (1.5MB .jpg) |
- Model of the SRP-FtsY complex, constructed using hydroxyl radical probing data as spatial constraints to position the 4.5S RNA and M domain in the complex as shown. FtsY and Ffh are depicted in the space-filling mode; FtsY, green; Ffh, blue; M domain, dark blue; signal peptide-binding site, dashed circle; 4.5S RNA, gold. Those BABE-Fe-modified positions that cleaved the 4.5S RNA are highlighted in contrasting colors, and their corresponding cleavage sites are indicated on the RNA in the corresponding colors (numbers in parentheses represent the analogous T. aquaticus residue numbers). White residues represent positions that failed to cleave the RNA.
- Location of the two BABE-Fe moieties at Ffh positions 153 and 344, where the cleaved nucleotides are highlighted in red. The cleavage pattern generated from these positions suggests placement of the BABE-Fe in the RNA major groove leading to strand scission in the two adjacent minor grooves (in this case one of the minor grooves is the RNA loop).
Reference:
- Spanggord, R.J., Siu, F., Ke, A. and Doudna, J.A. (2005) RNA-mediated interaction between the peptide-binding and GTPase domains of the signal recognition particle. Nat. Struct. Mol. Biol.12,1116-1122.(484KB .pdf)
© 2006 The Nature Publishing Group